Hemoglobin
Hemoglobin Principle Blood is mixed with an acid solution so tha t hemoglobin is converted to brown-colored acid hematin. This is then diluted with water till the brown color matches that of the brown glass standard. The hemoglobin value is read directly from the scale. Equipment (1) Sahli’s hemoglobinometer: This consists of Sahli’s graduated hemoglobin tube (marked in grams and percent) and a comparator with a brown glass standard. (2) Sahli’s pipette or hemoglobin pipette (marked at 20 μl or 0.02 ml). (3) Small glass rod (stirrer). (4) Dropping pipette. Reagents (1) N/10 hydrochloric acid (2) Distilled water Specimen: EDTA-anticoagulated venous blood or blood obtained by skin puncture. Method (1) Place N/10 hydrochloric acid into Sahli’s graduated hemoglobin tube up to the mark of 2 grams. (2) Take blood sample in Sahli’s pipette exactly up to 20 μl mark. Blood adhering to the exterior of the pipette is wiped away using absorbent paper or
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